pco.flim X – Fluorescence Lifetime Imaging Microscopy (FLIM) Camera
The latest pco.flim X by Excelitas, is the latest version of the first frequency domain (FD) Fluorescence Lifetime Imaging Microscopy (FLIM) camera using a fast modulatable CMOS sensor with two readout-taps per pixel. It offers improved cooling, which achieves -5°C with forced air cooling and -20°C image sensor temperature with water cooling. All of these features reduce the readout noise level below 15 [e-] and extend the usable exposure times to 4 instead of 2s. It can measure fluorescence lifetimes from 1 ns – 100 µs and therefore remains the frequency domain FLIM camera system with the largest resolution of 1008 x 1008 pixels and the highest maximum frame rate of 45 double images/s on the market.
The pco.flim X is the camera of choice for lightsheet fluorescence microscopy with fluorescence lifetime imaging, high-resolution fluorescence lifetime imaging in widefield microscopy and instant FRET measurements by using the donor fluorescence lifetime.